At the moment, the early indicators were being dismissed as particles, but the retrospective evaluation shown shared attributes with the problematic cells in society. Tracing the infection aided the lab establish additional laboratory treatments for screening and protection of assay-important mobile traces.
As samples are obtained on the Attune CytPix Flow Cytometer, the substantial-speed brightfield camera captures and retailers images of detected gatherings in a rate up to 6,000 images for every next, depending on the flow fee and impression measurement. For bigger overall flexibility, Attune Cytometric Software program helps you to change the graphic capture frequency as required and select a certain gate for image seize, if required.
(B) Uncooked 264.seven macrophages are sometimes annotated inaccurately with respect to particle depend due to their complexity and noncircular borders. The amount of singlets readily available for Assessment boosts when the consumer trains and employs a far more precise product.
Learn new insights into your experimental info by visualizing populations employing mixed fluorescence and morphology facts. Our software program interface wizards help downsampling, concatenation (merged documents) and dimensionality instruments which can be created into the software package for an intuitive, streamlined data Assessment knowledge.
"A picture isn't really worthy of 1000 dots, however it genuinely helps to see photos. It is great to see images that correlate with staining. By way of example, AnnexinV-good cells which have been morphologically various than nutritious cells, or CD14+ monocytes which are greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a skilled user has gated singlets confidently. Soon after analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates in excess of 4% aggregates.
Pair your Attune flow cytometer by having an autosampler for improved workflow performance, and add robotic integration for max throughput.
Our scientists will style and design panels for yourself as repeatedly you will need—cost-free, no merchandise obtain necessary.
The Attune NxT cytometer would be the trustworthy workhorse instrument your lab can trust in for consistent and trustworthy general performance. The Attune CytPix model features a significant-pace brightfield digicam and AI-driven impression Investigation application, with latest upgrades like machine Discovering resources and an intuitive person interface, enabling enhanced info precision, accuracy, and faster insights into sample biology.
Buyers can perspective occasion photographs in true-time to confirm sample excellent, optimize protocols, and document the morphology of each and every cell inhabitants recognized for Evaluation. Imaging capture also Gains from acoustic concentrating, which helps you to posture the cells in order that a pointy, centered impression is attained.
AI-driven automated impression Evaluation with the Attune Cytometric Application interprets event attributes into distinct morphology parameters that could be coupled with typical fluorescence and scatter parameters to expose hugely reproducible populace variations and distinctive subsets.
Application Notice: Automated graphic Investigation decreases user-to-person variability in flow cytometry gating strategies
Leverage the strength of Highly developed technological know-how and innovation to accelerate your discoveries. Believe in within the Attune Flow Cytometer relatives to deliver the trustworthiness, pace, and accuracy that established your function apart.
Adaptable payment system from Agilent Money Methods assist you to obtain the important instrumentation you require for speedy project, or to mature your lab.
At the guts of every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes audio waves to meticulously align cells inside the flow mobile. This progressive tactic surpasses conventional hydrodynamic methods, causing faster processing speeds, exceptional info quality, and enhanced sample throughput.
As the cell line experienced Beforehand been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the pictures and had been capable to document the microbial an infection no less than 5 days before.
Agilent Benefit Guarantee ensures you at the very least 10 years of instrument use within the date you purchase. Or else, We'll credit score you With all the residual price read more of the program toward an upgraded model.
In this article, we present that by gating strategies applying image-centered quantitative parameters can distinguish interacting cells from coincident activities to extra precisely analyze interacting cells.